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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/09/2022 |
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Actualizado : |
22/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
UMPIÉRREZ , A.; ERNST, E.; CARDOZO, A.; TORRES, A.; FERNÁNDEZ, M.; FRAGA, M.; VIGNOLI, R.; BADO, I.; VIDAL, R.; ZUNINO, P. |
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Afiliación : |
ANA UMPIÉRREZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; DÉBORAH ERNST, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ANDREA CARDOZO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; ALEXIA TORRES, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; MAGALÍ FERNÁNDEZ, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL VIGNOLI, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; INÉS BADO, Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.; ROBERTO VIDAL, Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago, Chile; Instituto Milenio de Inmunología e Inmunoterapia, Facultad de Medicina, Universidad de Chile, Santiago, Chile.; PABLO ZUNINO, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Non-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Austral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045 |
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Descripción física : |
SSN 0719-8132 (version on-line)
ISSN 0719-8000 (version print) |
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ISSN : |
0719-8132 (print); e-ISSN 0719-8000 (electronic) |
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DOI : |
10.4067/S0719-81322022000200045 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022.
Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy |
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Contenido : |
ABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics. MenosABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The ... Presentar Todo |
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Palabras claves : |
Antimicrobial resistance; Non-O157 STEC; PLATAFORMA EN SALUD ANIMAL; Shiga toxin subtypes. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16768/1/10.4067-s0719-81322022000200045.pdf
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Marc : |
LEADER 03084naa a2200325 a 4500
001 1063578
005 2022-09-22
008 2022 bl uuuu u00u1 u #d
022 $a0719-8132 (print); e-ISSN 0719-8000 (electronic)
024 7 $a10.4067/S0719-81322022000200045$2DOI
100 1 $aUMPIÉRREZ , A.
245 $aNon-O157 Shiga toxin-producing Escherichia coli with potential harmful profiles to humans are isolated from the faeces of calves in Uruguay.$h[electronic resource]
260 $c2022
300 $cSSN 0719-8132 (version on-line) ISSN 0719-8000 (version print)
500 $aArticle history: Received 12 October 2021; Accepted 30 December 2021; Published 09 May 2022. Corresponding author: Ana Umpiérrez; Avenida Italia 3318, CP 11600, Montevideo, Uruguay; aumpierrez@iibce.edu.uy
520 $aABSTRACT.- Shiga toxin-producing Escherichia coli (STEC) infections are responsible for acute illnesses and deaths in humans. Cattle and humans are exposed to STEC through faeces and contaminated food and water. The big six and O157 STEC serogroups are important food and water-borne human pathogens. Additionally, Stx1a, Stx2a and Stx2c subtypes are highly associated with the haemolytic uremic syndrome. This study aimed to determine Shiga toxin-subtypes, the presence of antigen 43 families, the genotypic and phenotypic antimicrobial susceptibility profiles, O-serogrouping, phylotypes and phylogenetic relatedness of STEC of calf origin. Sixteen STEC isolates from calf origin were analysed. PCR was performed to determine Stx subtypes, serogroups, the presence of ag43 I and IIand phylotypes. The antimicrobial profile was evaluated and the presence of PMQR and fosfomycin genes was determined by PCR. The clonal relatedness of STEC was studied by PFGE. The genotypes stx1a+c,stx1a+, stx1a+/stx2e+, stx1a+c/stx2e and stx2awere detected. Ag43 II was the most prevalent among subfamilies. STEC isolates were serotyped as O103 (n=5) and O111 (n=6). Fifty per cent of the isolates were classified as B1 phylogroup, 4/16 as E, 1/16 as C, and 1/16 as F. Non-O157 STEC isolates showed a high level of diversity, independent of the geographical and farm-origin. Isolates were resistant to ampicillin, ciprofloxacin, gentamicin, and fosfomycin-trometamol. The gene fosA7 was detected in 1 isolate. The virulence profiles, including Shiga toxin-subtypes and serogroups, denote the potential harm of non-O157 STEC isolates to humans. We also confirmed that circulating non-O157 STEC from cattle present genetic heterogeneity and are susceptible to antibiotics.
653 $aAntimicrobial resistance
653 $aNon-O157 STEC
653 $aPLATAFORMA EN SALUD ANIMAL
653 $aShiga toxin subtypes
700 1 $aERNST, E.
700 1 $aCARDOZO, A.
700 1 $aTORRES, A.
700 1 $aFERNÁNDEZ, M.
700 1 $aFRAGA, M.
700 1 $aVIGNOLI, R.
700 1 $aBADO, I.
700 1 $aVIDAL, R.
700 1 $aZUNINO, P.
773 $tAustral Journal of Veterinary Sciences, 2022, Vol. 54 Issue 2, p.45-53. doi: https://doi.org/10.4067/S0719-81322022000200045
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
26/04/2023 |
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Actualizado : |
19/06/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
REBOLLO, I.; SCHEFFEL, S.; BLANCO, P.H.; MOLINA, F.; MARTÍNEZ, S.; CARRACELAS, G.; AGUILAR, I.; PÉREZ DE VIDA, F.; ROSAS, J.E. |
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Afiliación : |
MARÍA INÉS REBOLLO PANUNCIO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Department of Statistics, College of Agriculture, Universidad de la República, Montevideo, Uruguay; SHEILA SCHEFFEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Department of Statistics, College of Agriculture, Universidad de la República, Montevideo, Uruguay; PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO MOLINA CASELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN MARTÍNEZ KOPP, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JULIO GONZALO CARRACELAS GARRIDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; IGNACIO AGUILAR GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Department of Statistics, College of Agriculture, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Consolidating twenty-three years of historical data from an irrigated subtropical rice breeding program in Uruguay. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Crop Science, 2023. https://doi.org/10.1002/csc2.20955 - [Article in Press]. |
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ISSN : |
1435-0653 |
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DOI : |
10.1002/csc2.20955 |
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Idioma : |
Inglés |
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Notas : |
Article history: First published 15 March 2023. -- Corresponding author: jrosas@inia.org.uy -- |
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Contenido : |
Breeding programs generate vast amount of data which are often scattered in separate files. This hinders the application of modern breeding tools such as multi-environment analyses and genomic selection. This research work describes the process of consolidating 23 years of phenotypic, pedigree, and genomic records from the Uruguayan national rice (Oryza sativa L.) breeding program, and the features and structure of the resulting database. Using a custom-made R code, we gathered all the available data from 1997 to 2020 corresponding to field trials, blast disease evaluation nurseries, laboratory analyses of milling and cooking quality, pedigree information, and genomic information for selected advanced breeding lines, and organized it into a relational database. Records of 996 trials in 12 locations over a span of 23 years, 91,636 field plots with information on 14 phenotypic variables, pedigree for 19,447 genotypes, and genomic information regarding 61,260 single nucleotide polymorphism (SNP) markers for 965 genotypes were recovered. The dataset is structured in trials, phenotypes, lines, genomic information, and SNP tables in an easy-to-access relational database, which will be a valuable resource for rice breeding. © 2023 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America |
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Palabras claves : |
Breeding program; Breeding trials; Phenotypic data. |
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Thesagro : |
ORYZA SATIVA L. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02322naa a2200301 a 4500
001 1064059
005 2023-06-19
008 2023 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $a10.1002/csc2.20955$2DOI
100 1 $aREBOLLO, I.
245 $aConsolidating twenty-three years of historical data from an irrigated subtropical rice breeding program in Uruguay.$h[electronic resource]
260 $c2023
500 $aArticle history: First published 15 March 2023. -- Corresponding author: jrosas@inia.org.uy --
520 $aBreeding programs generate vast amount of data which are often scattered in separate files. This hinders the application of modern breeding tools such as multi-environment analyses and genomic selection. This research work describes the process of consolidating 23 years of phenotypic, pedigree, and genomic records from the Uruguayan national rice (Oryza sativa L.) breeding program, and the features and structure of the resulting database. Using a custom-made R code, we gathered all the available data from 1997 to 2020 corresponding to field trials, blast disease evaluation nurseries, laboratory analyses of milling and cooking quality, pedigree information, and genomic information for selected advanced breeding lines, and organized it into a relational database. Records of 996 trials in 12 locations over a span of 23 years, 91,636 field plots with information on 14 phenotypic variables, pedigree for 19,447 genotypes, and genomic information regarding 61,260 single nucleotide polymorphism (SNP) markers for 965 genotypes were recovered. The dataset is structured in trials, phenotypes, lines, genomic information, and SNP tables in an easy-to-access relational database, which will be a valuable resource for rice breeding. © 2023 American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America
650 $aORYZA SATIVA L
653 $aBreeding program
653 $aBreeding trials
653 $aPhenotypic data
700 1 $aSCHEFFEL, S.
700 1 $aBLANCO, P.H.
700 1 $aMOLINA, F.
700 1 $aMARTÍNEZ, S.
700 1 $aCARRACELAS, G.
700 1 $aAGUILAR, I.
700 1 $aPÉREZ DE VIDA, F.
700 1 $aROSAS, J.E.
773 $tCrop Science, 2023. https://doi.org/10.1002/csc2.20955 - [Article in Press].
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